Jun 12 2008 10:49 AMMicroscopy & Microtechniques
VISUALISATION OF EXOCYTOSIS OF INSULIN-CONTAINING SECRETORY VESICLES IN PANCREATIC ß-CELLS USING TIRFM
One of life science's primary challenges is to
understand and define the role of a number
of proteins in cellular processes associated
with health and disease of living organisms.
Understanding this vast array of diverse
biochemical processes of the living cell is
paramount to not only gaining an insight into
the living organism as a whole, but also
discovering when and why these cellular
processes break down, thus causing disease.
Whilst innovative and specialised techniques
have been instrumental for many advances in
molecular biology, many questions concerning
the structure and function of cells can only be
answered by observing living cells. Live-cell
imaging offers the power of capturing the
dynamics of biological action in live cells and in
real-time, something not previously available
with biochemical approaches.
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