Direct PCR from a variety of plant species and tissue types is now possible with Kapa Biosystems new KAPA3G Plant PCR Kit, exclusively available in the UK from Anachem Ltd. This unique kit gives you fast, reproducible PCR with improved success rates and efficient amplification of long and difficult targets. It streamlines workflows for fast transgenic screening and you can work directly from leaf discs, seeds and crude plant extracts.
The secret to this revolutionary kit is the novel, third-generation (3G) DNA polymerase that brings great advantages over standard Taq enzyme, enabling higher performance PCR from whole plant tissue. Engineered via molecular evolution, the KAPA enzyme has been selected for improved tolerance to common plant-derived PCR inhibitors such as polyphenolics and polysaccharides. Kits are optimised for fast and efficient amplification of plant DNA from crude samples, DNA containing carry-over inhibitors from crude extraction methods, and purified DNA.