Feb 12 2010 01:00 PMLaboratory Products

Integral Supplement to E. coli O157 Isolation

 

The current UK outbreaks of Escherichia coli 0157 infection in young children have once again highlighted the seriousness of the ensuing illness, the importance of timely diagnosis and the need to track the source of infection. Enrichment of samples is an important part of the E. coli 0157 recovery and isolation process and Lab M’s Buffered Peptone Water is used with VCC Selective Supplement to prepare a pre-enrichment broth for organism recovery. It then also serves as a selective enrichment medium for the isolation of E. coli O157 from food and other samples.

As well as this enrichment broth, Lab M provides a full range of media for E. coli O157 culture, including modified Tryptone Soy Broth (mTSB), Sorbitol MacConkey Agar (SMAC) and Cefixime Tellurite MacConkey Agar (CT-SMAC), selective differential media for the isolation of E. coli O157:H7, the primary serovar associated with haemorrhagic colitis and haemolytic uraemic syndrome. Harlequin™ SMAC-BCIG is a modification of SMAC agar in which the addition of a specific chromogenic substrate (5-bromo-4-chloro-3-indoxyl-β-Dglucuronide [BCIG]) allows the straightforward differentiation of E. coli O157:H7 from other strains on the basis of colony colour.

Alongside these media is Rhamnose MacConkey (VTEC O26) Agar, selective for verocytotoxin producing E.coli O26, a strain that is also associated with haemorrhagic colitis and haemolytic uraemic syndrome. This medium is based on MacConkey agar, but substitutes rhamnose for the usual lactose as the fermentable carbohydrate. VTEC O26 colonies are unable to ferment the rhamnose present in the medium so remain translucent. All other non-O26 VTEC colonies appear as pink to red.

Request more information

You are requesting more details on: Integral Supplement to E. coli O157 Isolation.

Your details will be referred to Lab M and they will provide you with more information regarding this press release themselves. If you have not logged into the website, then please enter your details below.

Contact Details:

* Required fields

top of page Filed under: Laboratory Products

Reader Comments (Total 0 comments)

Post a comment

Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.

Post your comment:

top of page