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Mettler Toledo has launched an email series of tips and tricks to help researchers and scientists to improve pH measurements in laboratories. pH measurement is a frequent task in a lab, the results of which are often dependent on the performance of the instruments used. While there are regular check-ups and calibration possibilities for
pH meters, the measurements can also be influenced by external factors, such as the surrounding temperature or the age of the buffer solutions. As the company points out, there are cases where you measure the sensor and not the sample. Technique directly affects the success and repeatability of experiments and a few simple tips could
greatly improve pH measuring performance. For example, diaphragm clogging may occur and could damage standard pH equipment, as the reference junction on conventional pH electrodes can clog when TRIS reacts with silver ions in the filling solution. This may also occur with protein in the buffer, such as BSA, eventually causing slow or
fluctuating readings, or even entirely wrong results; an electrode can be selected that is specifically designed to be compatible with TRISbased buffers.
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