Mass Spectrometry & Spectroscopy

Quantification of Fluticasone Propionate and Salmeterol Xinafoate in Plasma at the Sub pg/mL Level Using UPLC/MS/MS  

Jul 09 2012

Author: Joanne Mather, Kendon Graham, Diego Rodriguez Cabaleiro, Stuart Chadwick, Paul Rainville, and Robert Plumb on behalf of Waters Corporation

Free to read

This article has been unlocked and is ready to read.


Sensitivity has been the driving force in the bioanalysis and DMPK arena for the last 15-20 years. The introduction of tandem quadrupole ESI mass spectrometry into the bioanalysis laboratory in the early 1990’s saw a step-change in the sensitivity and specificity of bioanalytical assays. Prior to this achieving sensitivities in the ng/mL range required complicated method development, elegant LC methods, elaborate solid-phase or liquid-liquid extraction processes to process large volumes of sample and even chemical modification of the analyte to impart better detection characteristic. The introduction of ESI tandem quadrupole MS changed the face of bioanalysis. Now sensitivities in the ng/mL range or could be achieved on virtually every molecule, from just a few hundred microlitres of sample without the need for complex sample preparation or derivitisation. Twenty years on and the pace of MS innovation and chromatographic science development means that sensitivities in the pg/mL range or below are now possible, even for the most challenging of molecules. 

Free to read

This article has been unlocked and is ready to read.


Digital Edition

International Labmate 48.6 - September 2023

September 2023

In This Edition Chromatography Articles - Faster and automated analysis of aroma compounds in cosmetics Mass Spectrometry & Spectroscopy Articles - Frequently Asked Questions (FAQ) abou...

View all digital editions


PTA - Chelyabinsk 2023

Sep 27 2023 Chelyabinsk, Russia

Asia Labex

Oct 05 2023 Gandhinagar, India

Lab Asia 2023

Oct 10 2023 Hybrid event

Drug Discovery 2023

Oct 18 2023 Liverpool, UK


Oct 23 2023 Beijing, China

View all events