Mass Spectrometry & Spectroscopy

Quantification of Fluticasone Propionate and Salmeterol Xinafoate in Plasma at the Sub pg/mL Level Using UPLC/MS/MS  

Jul 09 2012

Author: Joanne Mather, Kendon Graham, Diego Rodriguez Cabaleiro, Stuart Chadwick, Paul Rainville, and Robert Plumb on behalf of Waters Corporation

Free to read

This article has been unlocked and is ready to read.

Download

Sensitivity has been the driving force in the bioanalysis and DMPK arena for the last 15-20 years. The introduction of tandem quadrupole ESI mass spectrometry into the bioanalysis laboratory in the early 1990’s saw a step-change in the sensitivity and specificity of bioanalytical assays. Prior to this achieving sensitivities in the ng/mL range required complicated method development, elegant LC methods, elaborate solid-phase or liquid-liquid extraction processes to process large volumes of sample and even chemical modification of the analyte to impart better detection characteristic. The introduction of ESI tandem quadrupole MS changed the face of bioanalysis. Now sensitivities in the ng/mL range or could be achieved on virtually every molecule, from just a few hundred microlitres of sample without the need for complex sample preparation or derivitisation. Twenty years on and the pace of MS innovation and chromatographic science development means that sensitivities in the pg/mL range or below are now possible, even for the most challenging of molecules. 

Free to read

This article has been unlocked and is ready to read.

Download


Digital Edition

Lab Asia 29.6 Dec

November 2022

In This Edition Chromatography Optimising Viral Vector Purification Strategies with Multimodal Chromatography Key UHPLC Characteristics Required for High throughput LC-MS New Low Volum...

View all digital editions

Events

CPhI & P-MEC China 2022 - NEW DATES

Dec 20 2022 Shanghai, China & Online 21 November 2022 to 13 January 2023

Smart Factory Expo 2023

Jan 25 2023 Tokyo, Japan

Arab Health

Jan 30 2023 Dubai, UAE

Nano Tech 2023

Feb 01 2023 Tokyo, Japan

Medlab Middle East

Feb 06 2023 Dubai, UAE

View all events