Microscopy & Microtechniques
THE USE OF HIGH-PRESSURE FREEZING IN CORRELATIVE LIGHT ELECTRON MICROSCOPY
Aug 09 2007 Read 1467 Times
With the emergence of Green Fluorescent Protein (GFP) light microscopy has switched from mainly static immuno fluorescence images into imaging very dynamic processes. Dynamic imaging is impossible in the electron microscope but it is excellent for high-resolution studies. Combining both advantages in techniques collectively called Correlative Light Electron Microscopy (CLEM) has gained increasing interest in recent years. New developments within this field include probe development, probe detection, and fixation. Some of these new developments will be discussed and a particular focus will be placed on the fixation process for electron microscopy.
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