How to Overcome the Limits of HPLC with Ion Chromatography
Nov 21 2019 Read 1156 Times
What’s the difference between High Pressure Liquid Chromatography (HPLC) and Ion Chromatography (IC)? What are the capabilities and limitations of each technique? Knowing these differences can actually make a huge difference. As a proven, robust and highly versatile technique, IC can help HPLC users extend their analytical capabilities tremendously. Metrohm’s free white paper compares the two methods systematically focusing on the strengths of ion chromatography.
HPLC is one of the most powerful analytical techniques in the modern laboratory. From research to manufacturing to legal, it is widely used in many sectors. However, there are some inherent limitations. Ionic and polar compounds pose a challenge. So do organic acids. Moreover, some analytes such as, e.g., fluoride and chloride are not active in the UV range, making it impossible to determine them with a UV detector, the detection technique most commonly used with HPLC. Ion chromatography (IC), on the other hand, is ideal for the separation and detection of ions and polar molecules, both organic and inorganic. This makes IC the ideal extension of HPLC increasing the analytical capabilities of the modern laboratory tremendously.
Over the last 30+ years, Metrohm has made Ion Chromatography a powerful tool for both research and routine analysis. Laboratories in the pharma, food, and water sectors in particular benefit not only from the method itself but from many automated inline sample preparation and injection techniques. Samples with a high load of particles (e.g., wastewater), solid and even gaseous samples can be analysed fully automatically down to the µg/L range with superior reproducibility. Hundreds of IC applications are available free of charge from the Metrohm Application Finder and users are supported in more than 120 countries by application specialists from the local Metrohm sales organisations.
Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.
In This Edition Mass Spectrometry & Spectroscopy - Translating Proteomic MALDi Tissue Typing to Clinical Pathology - Fusion Protein Complexes Analysed by CG-MALS - Non-equivalent, Multivalen...
View all digital editions
Jan 25 2020 San Diego, CA, USA
Jan 27 2020 Dubai, UAE
Jan 29 2020 Tokyo, Japan
Feb 03 2020 Dubai, UAE
Feb 12 2020 Tokyo, Japan