Chromatography

  • New System Provides High Efficiency Multi-Sample Prep Processing
    The new Nexera Prep Purification LC System improves productivity tremendously.

New System Provides High Efficiency Multi-Sample Prep Processing

Feb 07 2020 Read 346 Times

The Nexera Prep Series consists of multiple related products with the LC units as the core. Users select a pump, autosampler and detector to build a system according to the purpose of the preparative work. Systems can be configured using related products such as the LH-40 Liquid Handler, FRC-40 Fraction Collector, Shim-pack Scepter LC Column for Analysis and Preparative Work, and Column Hub.

Preparative purification is generally performed following a multi-step workflow, which requires experience and time:

  • Selection of system configuration and preparative column size based on the amount of purified sample or injection volume required
  • Selection of fraction collector and container size according to the intended purpose
  • Investigation of separation conditions in analytical scale
  • Optimisation of preparative LC conditions
  • Confirmation of the purity of collected fractions

In addition to these steps, in some cases salts need to be removed or solvent needs to be evaporated after purification, requiring additional time and effort.

The Nexera Prep Series reduces the time spent on examining conditions using the software’s fractionation simulation function. It also enables simplified, fast operation in transition from analytical to preparative scale, with a column portfolio that covers a wide range of sizes. Furthermore, Shimadzu’s unique UFPLC (Ultra Fast Preparative and Purification LC) high-purity purification system reduces the time required for powderisation via the elution of desalted target components with an organic solvent. This product satisfies a number of user demands, such as efficient desalting and powderisation, multi-sample and multi-fraction analysis, effective separation at low cost and the ability to check the purity.

Specifically, the product can be used for the following purposes:

  • Concentration and purification of immunosuppressive agents
  • Preparative work for impurities in degradants of medicinal agents
  • Separation and purification of functional components from natural materials
  • Consecutive analysis to separate and purify polymers and their additives and confirm the purity

With the fractionation simulation function of the LabSolutions analysis software and the Shim-pack Scepter LC column for analytical and preparative HPLC, which offers excellent scalability, the product reduces the time required for investigating preparative work conditions. The LH-40 Liquid Handler can be used both, as a fraction collector and as an autosampler for large-volume injection with liquid surface detection function (option). It also enables seamless purity checks through re-injection (option) of collected sample after fractionation. Additional features including a sample rescue protocol to collect the sample from the system if an error occurs after an injection, a function to automatically extract the target peaks only, and high-purity preparative purification using detector signals from up to four channels, enable more efficient operation.

Samples must be in a state of concentrated solution or dry powder to analyse their structure or to store them. When a solution contains water, it takes time to dry and concentrate it. When a salt such as trifluoroacetate has been added, it remains in the crystal of the compound, lowering the purity and negatively influencing pharmacokinetic studies and other tests. Shimadzu’s unique, automated UFPLC purification system introduces fractions containing the target compound, separated in a preparative column, into a trap column. Salt and water are then washed off and elution is induced with an organic solvent. This reduces the time required for drying, providing compounds with a high level of purity.

Both the LH-40 Liquid Handler and FRC-40 Fraction Collector are designed to save installation space, requiring only half the space of other vendors’ products. They support containers of a form and size suitable for the recovered quantity. Up to six units can be connected, so up to 3240 10 mm diameter test tubes can be placed. Using the new Column Hub, up to six columns and four flow-line switching valves can be installed, enabling the compact configuration of various systems.

More information online

Reader comments

Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.

Post a Comment




Digital Edition

International Labmate February 2020

February 2020

In This Edition Chromatography - The Purification of Crown Ether Samples by a Quaternary Solvent Flash Chromatography System - New Wettable Phase Thrives in Water - Reduced Surface Activity...

View all digital editions

Events

SIAF Guangzhou

Feb 26 2020 Guanghzou, China

PITTCON 2020

Mar 01 2020 Chicago, IK, USA

CISILE 2020

Mar 04 2020 Beijing, China

Medical Fair India 2020

Mar 05 2020 New Delhi, India

China Lab 2020

Mar 11 2020 Guangzhou, China

View all events