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Pipette Calibration and Technique

Feb 14 2019 Read 181 Times

Calibrated pipettes and carefully followed pipette protocols produce repeatable results in the lab. Recent scientific literature offers a constant stream of reminders that pipettes and pipetting technique continues to play a significant role in the success or failure of an experiment.

This review focuses on information from genomics and proteomics papers published in recent years. It provides examples of risks associated with inattention to calibration and technique and includes recommended pipetting protocols and calibration standards.

When pipette calibration and user technique receive the focus they need, irregularities arising from mechanical and human variables are significantly reduced.

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