Laboratory Products

  • Innovative 2D-Gradient for Enhanced PCR Optimisation

Innovative 2D-Gradient for Enhanced PCR Optimisation

May 28 2020 Read 159 Times

Optimising the annealing temperature to improve PCR results is a routine task, so why not optimise as far as you can? More and more findings indicate that optimisation of the denaturation temperature is worthwhile as well. A high denaturation temperature will harm the enzyme and other biomolecules in your assay. A low denaturation temperature may result in inefficient splitting of the complimentary DNA strands, as is often found with GC-rich templates or templates prone to form ‘hairpin structures’.

Eppendorf’s new 2D-Gradient allows optimisation of the annealing and the denaturation temperature in a single run – taking less time than ever before. This gives you the ideal set of temperatures for your PCR assay - quickly, conveniently, reliably.

Read the application note.

More information online

Reader comments

Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.

Post a Comment





Digital Edition

Lab Asia June 2020

June 2020

In This Edition Articles Chromatography - What is Eluent Generation? - Automated online μSPE Clean-up for QuEChERS Approach to Pesticides Analysis - Simple and Seamless Routine Analysis...

View all digital editions

Events

Korea Lab 2020 - NEW DATES

Jul 27 2020 Kintex, South Korea

M&M 2020 - ONLINE EVENT

Aug 02 2020 Milwaukee, Wisconsin, USA

DXC 2020 - ONLINE EVENT

Aug 03 2020 Lombard, IL, USA

IE Expo China 2020 - NEW DATES

Aug 13 2020 Shanghai, China

ACS National Meeting & Expo, Fall 2020

Aug 16 2020 San Francisco, CA, USA

View all events