Laboratory Products
Innovative Kits for Large-ScalePlasmid DNA Isolation
May 02 2008
purification capacity is up to 2.5 mg of plasmid DNA for the Mega Kit and up to 10 mg for the Giga Kit. With commonly used techniques, isolation of large quantities of plasmid DNA was often very time consuming, requiring as much as several hours to complete. This posed significant challenges for researchers facing increasing time pressures. In contrast, the new kits allow isolation of plasmid DNA in less than 50 minutes due to a vacuum-driven protocol. In addition, by using the companyâs QIAvac 24 Plus vacuum device in combination with the new QIAvac Holder, it is possible to process up to 12 preparations in parallel. Virtually no hands-on work is required during the entire procedure. Another advantage of the CompactPrep Plasmid Mega and Giga kits is the highly concentrated, low-endotoxin plasmid DNA produced. The new kits use small columns that enable elution of DNA in a very low buffer volume, resulting in DNA concentrations of up to 2.5 ìg/ìl. In addition, by using a supplemental wash buffer, endotoxin levels are usually lower than 1 EU/ìg DNA. The resulting purified plasmid DNA is suitable for all routine downstream applications, including cloning, enzymatic restriction, in vitro transcription and translation. Due to the kitsâ proprietary wash buffer, the extracted plasmid DNA is ideal for transfection experiments, even with cell lines as sensitive as Huh-7.
Digital Edition
Lab Asia 31.2 April 2024
April 2024
In This Edition Chromatography Articles - Approaches to troubleshooting an SPE method for the analysis of oligonucleotides (pt i) - High-precision liquid flow processes demand full fluidic c...
View all digital editions
Events
Apr 25 2024 Istanbul, Turkey
Apr 28 2024 Montreal, Quebec, Canada
May 05 2024 Seville, Spain
InformEx Zone at CPhl North America
May 07 2024 Pennsylvania, PA, USA
May 14 2024 Oklahoma City, OK, USA