Laboratory Products

  • Innovative Kits for Large-ScalePlasmid DNA Isolation

Innovative Kits for Large-ScalePlasmid DNA Isolation

May 02 2008 Read 2652 Times

Two new products for large-scale plasmid DNA isolation are being introduced by QIAGEN. The CompactPrep Plasmid Mega and Giga kits offer greater convenience and faster extraction than other methods. The
purification capacity is up to 2.5 mg of plasmid DNA for the Mega Kit and up to 10 mg for the Giga Kit. With commonly used techniques, isolation of large quantities of plasmid DNA was often very time consuming, requiring as much as several hours to complete. This posed significant challenges for researchers facing increasing time pressures. In contrast, the new kits allow isolation of plasmid DNA in less than 50 minutes due to a vacuum-driven protocol. In addition, by using the company’s QIAvac 24 Plus vacuum device in combination with the new QIAvac Holder, it is possible to process up to 12 preparations in parallel. Virtually no hands-on work is required during the entire procedure. Another advantage of the CompactPrep Plasmid Mega and Giga kits is the highly concentrated, low-endotoxin plasmid DNA produced. The new kits use small columns that enable elution of DNA in a very low buffer volume, resulting in DNA concentrations of up to 2.5 ìg/ìl. In addition, by using a supplemental wash buffer, endotoxin levels are usually lower than 1 EU/ìg DNA. The resulting purified plasmid DNA is suitable for all routine downstream applications, including cloning, enzymatic restriction, in vitro transcription and translation. Due to the kits’ proprietary wash buffer, the extracted plasmid DNA is ideal for transfection experiments, even with cell lines as sensitive as Huh-7.

Reader comments

Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.

Post a Comment

Digital Edition

Labmate UK & Ireland September 2020

September 2020

In This Issue Articles - Review of China market shows real potential for lab companies - Biphenyl Modified Silica Stabilised by Bulky Substituents Laboratory Products - ChIP Kits for H...

View all digital editions


ISC 2020 - POSTPONED TO 2022

Sep 21 2020 Budapest, Hungary

Analitika Expo 2020 - NEW DATES

Sep 22 2020 Moscow, Russia

West Africa Water Expo

Oct 06 2020 Lagos, Nigeria


Oct 06 2020 Digital Forum

View all events