Figure 1: The Restek Biphenyl LC column's ability to separate isobars and critical pairs in drug panels made it possible to include more compounds per panel, leading to improved accuracy for these critical assessments.
Figure 2: The unique selectivity of the Raptor Biphenyl column is well-suited for this analysis of pharmaceuticals and personal care products by UHPLC.
Figure 3: Excellent separation and peak shapes were achieved in this multi-mycotoxin analysis using a Raptor Inert Biphenyl LC column.

The Original Biphenyl: First and Still the Best

Jun 03 2025

Twenty years ago, Restek was the first to bring chromatographers the pioneering Restek Biphenyl LC column. Trailblazing a new approach to LC separations, the Restek Biphenyl LC column's exceptionally unique selectivity made it possible to achieve better separations than were possible on traditional C18 columns or other phenyl chemistries. Today, as copycat columns continue to come out, Restek remains committed to innovation, not imitation. Keep reading to learn why innovation matters and how our Biphenyl LC column can help simplify your separations and advance your science today.

Want to learn how the Biphenyl column changed chromatography? Read the Biphenyl story now→

✓ Unique selectivity does everything a C18 column does and more.
✓ Excellent separation of aromatic compounds and positional isomers.
✓ Go-to choice in method development and for large panel analyses.

Why Innovation Matters

The innovative Biphenyl (USP L11) is Restek’s most popular LC stationary phase because it is particularly adept at separating compounds that are hard to resolve or that elute early on other popular phases. As a result, our rugged Biphenyl LC columns are extremely useful for fast separations—even of isobars—in challenging analyses, especially those that require a mass spectrometer (MS). The Restek Biphenyl LC column's heightened selectivity makes it a go-to choice as a starting column in method development and eliminates the need for complex mobile phases that are not well suited for MS detection.

Interested to see how Biphenyl can work in your lab? Get in touch with an expert today→

Choose the Right Biphenyl LC Column for Your Analysis

Raptor SPP Columns Premier performance columns that combine the speed of superficially porous particles (SPP or “core-shell”) with time-tested Restek Biphenyl selectivity.

Force FPP Columns Long-lasting, reproducible, and fully scalable FPP (fully porous particle) columns that let you easily transfer methods across your entire lab, from trusted-but-aged HPLCs to cutting-edge UHPLCs.

Ultra Columns High-purity, type-B silica that minimizes activity and creates high-density bonding for reliable HPLC use.