Microscopy & Microtechniques
New Dedicated TIRF Microscopy Objectives for Live Cell Surface Observations
Mar 13 2006 Read 1697 Times
Objectives with high NA are required for TIRFM. High NAs allow the laser light to attain the critical angle required for total internal reflection at the cover slip-medium interface. This induces an evanescent field which only excites fluorophores at the interface. Unlike standard widefield microscopy, the light does not travel through specimens, reducing photo-damage and enabling cells to live longer. A higher NA enables a shallower laser light angle and reduces penetration depth of the evanescent field.
The APO 100xOHR holds the world record for light efficiency and resolution due to its unparalleled NA of 1.65. With this objective, the penetration depth of the evanescent field can be reduced below 100nm, providing an exceptionally fine depth of focus for highly precise observation of the cell surface and membrane-associated processes. This is achieved through a very high signal-to-background ratio and a z-resolution that is not even achieved by confocal microscopy. This is possible due to the total absence of any out-of-focus fluorescence, as excitation only occurs in the plane of observation directly adjacent to the cover slip-medium interface.
Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.
In This Edition Articles - Translating Proteomic MALDi Tissue Typing to Clinical Pathology - Fusion Protein Complexes Analysed by CG-MALS - The Separation and Purification of Glutamine Deri...
View all digital editions
Nov 25 2019 Beijing, China
Dec 05 2019 New Delhi, India
Dec 13 2019 Kathmandu, Nepal
Jan 25 2020 San Diego, CA, USA
Jan 27 2020 Dubai, UAE