Laboratory Products

  • Optimal Filter Performance for Quantitative Polymerase Chain Reaction 

Optimal Filter Performance for Quantitative Polymerase Chain Reaction 

Jun 25 2020 Read 235 Times

Optical filters play a crucial role in detecting the corona pathogen SARSCoV2. With the expanded Ultra series, Laser Components now offers optical filters that were specifically developed for real-time qPCR tests for the SARS-CoV-2 virus. Patient samples can thus be analysed faster and more efficiently. 

The polymerase chain reaction (PCR) is a proven method in which the inactive genetic material of viruses is exponentially multiplied so that it can finally be detected in the laboratory. In quantitative real-time PCR, additional fluorescent dyes are added to the reaction, which become active at a specific point in time during the chain reaction. This makes both quantitative analysis and detection possible. The performance of this method depends heavily on how accurately the fluorescence wavelength is detected. Bandpass filters that suppress all other wavelengths ensure this. 

 

Blocking that is greater than OD6 results in bandpass filters that have a transmission in the pass band of more than 95% and a medium wavelength tolerance of less than ± 0.3% at the required wavelength to increase qPCR efficiency. These filter sets are optimally adapted for use with fluorescent dyes such as FAM, Cy5, HEX, JOE, ROX, TexasRed, and TAMRA.  

Not only manufacturers of medical PCR instruments will be able to benefit from them. 

More information online

Reader comments

Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.

Post a Comment





Digital Edition

Lab Asia June 2020

June 2020

In This Edition Articles Chromatography - What is Eluent Generation? - Automated online μSPE Clean-up for QuEChERS Approach to Pesticides Analysis - Simple and Seamless Routine Analysis...

View all digital editions

Events

Korea Lab 2020 - NEW DATES

Jul 27 2020 Kintex, South Korea

M&M 2020 - ONLINE EVENT

Aug 02 2020 Milwaukee, Wisconsin, USA

DXC 2020 - ONLINE EVENT

Aug 03 2020 Lombard, IL, USA

IE Expo China 2020 - NEW DATES

Aug 13 2020 Shanghai, China

ACS National Meeting & Expo, Fall 2020

Aug 16 2020 San Francisco, CA, USA

View all events