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High-Performance Liquid Chromatography (HPLC) is a powerful analytical technique used to separate, identify, and quantify components in a complex liquid mixture. It operates under high pressure to push the liquid sample through a column packed with fine particles, resulting in efficient separation of compounds based on their chemical interactions with the stationary phase. HPLC is widely employed in pharmaceuticals, environmental testing, food safety, and biotechnology, offering high sensitivity, accuracy, and the ability to handle complex samples with precision.

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