• New ?chromatography Flow-mode? Option Transforms Zetasizer Nano 
Into In-line Detector for Absolute Size Exclusion Chromatography

Chromatography

New ?chromatography Flow-mode? Option Transforms Zetasizer Nano Into In-line Detector for Absolute Size Exclusion Chromatography

May 02 2007

Launched at Pittcon 2007 the new ?chromatography flow-mode' option for Malvern Instruments' market-leading Zetasizer Nano particle and molecular characterisation system, enables its use as an in-line chromatography detector for protein applications.

A significant new development, ?chromatography flow-mode' permits the coupling of dynamic light scattering with size exclusion chromatography, conferring the ability to perform Absolute Size Exclusion Chromatography (ASEC). This allows protein size to be measured directly rather than relying on column calibration or additional data from a second detector, and means that molecular weight standards are no longer required to identify oligomeric species or determine protein purity.

To use the Zetasizer Nano as an absolute light scattering detector it simply needs to be connected after the last detector in a size exclusion chromatography (SEC) system. The instrument can be added to any SEC set-up; no column calibration is required and the Zetasizer Nano's software makes the entire measurement process simple and straightforward. For the majority of protein separations in aqueous media, no extra parameters are required. Size is plotted in real time as material is eluted from the column, and the molecular weight of each peak is calculated automatically at the end of the measurement.

The Zetasizer Nano was designed specifically to meet the low concentration and small sample volume requirements of protein and other biomolecular applications and is now in widespread use. It is by applying the system's patented optical design and high sensitivity capabilities to real time flow measurements that Malvern has facilitated the Zetasizer Nano's use as a chromatography detector, providing a major new tool for protein researchers.

Digital Edition

ILM 49.5 July

July 2024

Chromatography Articles - Understanding PFAS: Analysis and Implications Mass Spectrometry & Spectroscopy Articles - MS detection of Alzheimer’s blood-based biomarkers LIMS - Essent...

View all digital editions

Events

ADLM 2024

Jul 28 2024 San Diego, CA USA

InaLab 2024

Jul 30 2024 Jakarta, Indonesia

Miconex

Jul 31 2024 Chengdu, China

ACS National Meeting - Fall 2024

Aug 18 2024 Denver, CO, USA

EMC2024

Aug 25 2024 Copenhagen, Denmark

View all events