New Solutions for Rapid, Sensitive Isolation of E. Coli O157
Nov 07 2006
E. coli O157 is one of the most important foodborne pathogens of animal origin. Associated primarily with self-limiting diarrhoea, it can also lead to much more serious conditions.
Low numbers, or overgrowth of E.coli O157 colonies by competing flora, can make the organism difficult to detect. Lab M's recommended protocol for enrichment and isolation uses both HAL006 medium and Captivate? O157.
Six-hour enrichment in modified Tryptone Soy Broth (LAB165) at 42ÂºC is followed by immunomagnetic separation using Captivate? O157 particles and plating to HAL006, with or without the addition of cefixime tellurite supplement. E. coli O157:H7 appear as pale translucent colonies on this medium. Their identity can be confirmed using latex agglutination kits or appropriate antisera.
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