Microscopy & Microtechniques

Antibiotic Resistance - Nanotech to the Rescue: Nanomechanical detection of drug-target interactions

Jan 27 2010

Author: Dr Mike Fisher & Dr David Sarphie on behalf of Bio Nano Consulting

Free to read

This article has been unlocked and is ready to read.


Drug resistance has evolved from being an infrequent and manageable occurrence in the treatment of microbial infections, to a healthcare problem on a global scale. The last five years has seen a meteoric rise in
the cases of life-threatening infections by mutated forms of common bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE). Eradication of such multi-drug resistant (MDR) strains is proving difficult since there are not enough effective antimicrobials on the market, and very few in the development pipelines. There is though, a concerted effort to develop new compounds capable of combating these infections effectively. A novel drug-target binding detection system is proving itself to be a big hit in this search. It is based on micro-cantilever technology and provides exquisite information on the nature
of a drug-target interaction. Furthermore, it can be applied on a high throughput basis making the search for new products much quicker too.

By immobilising a ligand on one side of a cantilever and adding the relevant receptor in solution, the cantilever bends in response to the change in surface stress when the ligand and receptor interact. This nanomechanical biosensing transduction mechanism has been used for measuring a number of biochemically specific interactions such as sequence-specific DNA hybridisation, single base mismatches, DNA quadruplex, protein recognition and has also been used for the detection of interferon-alphainduced I-8U gene expression in total human RNA - a potential marker for melanoma progression and viral infections. This technology has now been applied very successfully to quantify drug–target binding interactions.

Free to read

This article has been unlocked and is ready to read.


Digital Edition

ILM 49.5 July

July 2024

Chromatography Articles - Understanding PFAS: Analysis and Implications Mass Spectrometry & Spectroscopy Articles - MS detection of Alzheimer’s blood-based biomarkers LIMS - Essent...

View all digital editions


ADLM 2024

Jul 28 2024 San Diego, CA USA

InaLab 2024

Jul 30 2024 Jakarta, Indonesia


Jul 31 2024 Chengdu, China

ACS National Meeting - Fall 2024

Aug 18 2024 Denver, CO, USA


Aug 25 2024 Copenhagen, Denmark

View all events