• Solving HPLC Throughput Constraints Imposed by Limited Sample Solubility

Chromatography

Solving HPLC Throughput Constraints Imposed by Limited Sample Solubility

Mar 05 2009

Dynamic Extractions has recently extended its range of HPCCC instruments by adding the Spectrum HPCCC. This instrument features both an analytical coil/column for scouting and method development, and a second coil/column for semi-prep and prep separations. In many preparative scale HPLC processes, throughput is ultimately limited by sample solubility. In comparison to HPLC, where liquid and solid (respectively mobile and stationary) phases are used, HPCCC instruments allow the use of liquids as both stationary and mobile phases. Such systems have inherent advantages of higher capacity and improved solubilisation and injection characteristics.  Rather than the solubility of samples being a limiting factor, it tends towards insignificance since the sample can be injected onto the column in either the mobile or stationary phase or a mixture of both. The sample can also be loaded as either a solution or a suspension, without causing blockages or affecting chromatographic performance.


The Spectrum HPCCC instrument has been specifically designed and developed to provide solutions to problems in discovery and development applications. This instrument allows the separation of samples, including those that are difficult to solubilise, firstly at milligram scale to develop purification methods, and then to scale those methods up, allowing the purification of 1-2 gram crude material per injection. This bench scale instrument allows upwards of 20 grams of crude material to be processed every 8 hours. It does not end there: other instruments in the range allow the same separation to be scaled easily and speedily to preparative and pilot plant scales.


Digital Edition

Lab Asia 31.2 April 2024

April 2024

In This Edition Chromatography Articles - Approaches to troubleshooting an SPE method for the analysis of oligonucleotides (pt i) - High-precision liquid flow processes demand full fluidic c...

View all digital editions

Events

FORUMESURE

Apr 22 2024 Marrakech, Morroco

Korea Lab 2024

Apr 23 2024 Kintex, South Korea

Korea Chem 2024

Apr 23 2024 Seoul, South Korea

Lab Indonesia

Apr 24 2024 Jakarta, Indonesia

View all events