• The Swiss Knife of Analytics


The Swiss Knife of Analytics

Oct 07 2022

The next era of SFC

Supercritical fluid chromatography (SFC) is a separation method that uses supercritical carbon dioxide (CO2) as the mobile phase. SFC technology has existed for a long time, but only due to recent advancements in SFC instrument and column technologies, it is now beginning to be used for routine analysis in academia, as well as industrial labs. By using supercritical CO2, which can be mixed with water-soluble solvents despite its low polarity, SFC offers a powerful, complimentary method that is different from conventional GC and HPLC separations. Supercritical fluids have a higher coefficient of diffusion with lower density and viscosity than liquids. Higher diffusivity results in better distribution into the mobile phase and higher separation efficiency, while lower viscosity allows for higher flow rates, without the pressure limitation of HPLC, offering the possibility of ultra-fast analytical runs.

Modern SFC systems are comparable to HPLC systems in configuration and operation. There are only three major differences to be taken into account:

  1. A solvent delivery pump designed specifically for pumping supercritical CO2 is required.
  2.  A dedicated unit, called a backpressure regulator (BPR), is required to keep the CO2 in a solvent state (liquid or supercritical fluid) preventing it from vaporising by maintaining the pressure within the flow path on a constant level. In an SFC system, the BPR unit is positioned downstream from a UV or PDA detector or upstream from an evaporative light scattering detector (ELSD) or mass spectrometer (MS).
  3. If an ELSD or MS unit is used for detection, then an additional ‘Make-up’ solvent pump is required to prevent precipitation in the flow channels or to improve sensitivity during MS detection, because supercritical carbon dioxide does not promote ionisation.

Shimadzu’s Nexera UC supercritical fluid chromatograph offers unique hardware innovations to ensure reliable and stable analysis. Adding the specificity of MS detection to the versatility of SFC makes it an ideal tool for challenging sample separations.

To enable customer- and application-specific solutions in the pharmaceutical, chemical and food industries, the Nexera UC supercritical fluid chromatography system is available in multiple configurations.

‘Green’ chromatography

Gas cylinders are filled with CO2 generated by industrial plants, no new CO2 is generated for operating SFC. On the other hand, organic solvents as used in standard HPLC applications are incinerated for disposal, which emits large amounts of energy and CO2. Therefore, switching a separation method from HPLC to SFC reduces cost and the environmental impact of an analysis.

More information online

Digital Edition

ILM 49.5 July

July 2024

Chromatography Articles - Understanding PFAS: Analysis and Implications Mass Spectrometry & Spectroscopy Articles - MS detection of Alzheimer’s blood-based biomarkers LIMS - Essent...

View all digital editions


ADLM 2024

Jul 28 2024 San Diego, CA USA

InaLab 2024

Jul 30 2024 Jakarta, Indonesia


Jul 31 2024 Chengdu, China

ACS National Meeting - Fall 2024

Aug 18 2024 Denver, CO, USA


Aug 25 2024 Copenhagen, Denmark

View all events