Chromatography

  • New Size Exclusion Columns for UHPLC

New Size Exclusion Columns for UHPLC

May 12 2016 Read 1138 Times

TSKgel UP-SW3000, a new 2 micron stationary phase, is the latest addition to the renowned TSKgel SW series of silica based gel filtration columns from Tosoh Bioscience. Packed into UHPLC column hardware it supports a smooth transfer of existing HPLC methods to UHPLC technology. TSKgel UP-SW3000 features the same surface modification and pore size as the well-established TSKgel G3000SWXL being the gold standard for QC of therapeutic antibodies in Biopharma for decades. Over 40 years of experience in developing and producing SEC stationary phases are incorporated into the new series of UHPLC columns for the analysis of proteins in the range of 10 to 500 kDa.

Methods developed on conventional gel filtration columns can easily be transferred to UHPLC technology when using TSKgel UP-SW3000. The 30 cm column delivers increased resolution between mAb fragments, monomers, and aggregates.  A 15 cm column is available to shorten analysis time while maintaining resolution. To achieve maximum resolution and fully exploit the potential of the columns the use of a UHPLC system is highly recommended.  A so-called DC (direct connect) guard column can be attached directly to the analytical column to minimise extra column band broadening.

TSKgel SW columns stand out from other silica-based high performance size exclusion columns by virtue of their large pore volumes. They are based on highly porous, ultra-pure silica particles, the surface of which has been shielded from interacting with proteins by applying a proprietary surface chemistry. This ensures highest recoveries and accurate quantitation. The validated manufacturing and packing process delivers a reliable batch-to-batch reproducibility.

Read comments0

Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.


Digital Edition

Lab Asia February 2019

February 2019

In this edition Articles - Detection of molecular markers in aquatic sediments by ion profi les obtained by GC/MS system - Fighting the Resistance: How Rapid Microbial ID with MALDI MS and A...

View all digital editions

Events

Biotech World

Feb 25 2019 Moscow, Russia

China Lab 2019

Feb 26 2019 Guangzhou, China

SmartLab Exchange

Feb 27 2019 Berlin, Germany

IFPAC Annual Meeting

Mar 03 2019 Maryland, (Washington DC), USA

Forensics Europe Expo

Mar 05 2019 London, UK

View all events