• Upgrade your HILIC Application to UHPLC

Upgrade your HILIC Application to UHPLC

May 14 2015 Read 1304 Times

TSKgel Amide-80, renowned for analysing protein glycosylation, is now available in 2 µm particles, ideally suited for high resolution UHPLC.

TSKgel Amide-80 columns packed with 2 µm silica based particles are the latest additions to the well-known TSKgel Amide-80 series from Tosoh Bioscience.  The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction (HILIC) mode of chromatography.  The Amide-80 phase shows higher retention of polar compounds than other Amide columns on the market.

For years, TSKgel Amide-80 columns have been used successfully for HILIC separations of polar compounds such as saccharides, glycans, oligosaccharides or peptides, documented in more than 250 scientific publications. In parallel to the growth of the biotherapeutics market the use of Amide-phases for glycosylation analysis steadily increased.

Packed with spherical silica particles that are covalently bonded with non-ionic carbamoyl groups, TSKgel Amide-80 provides higher stability than conventional amino-phases and a unique selectivity. The characterisation of the new 2 µm version of TSKgel Amide-80 compared to the renowned 3 µm Amide-80 based on the system proposed by Y. Kawachi et al. (J. Chromatogr. A, 1218 (2011) 5903 ff) proves the similarity in selectivity.

Reader comments

Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.

Post a Comment

Digital Edition

Lab Asia August 2019

August 2019

In this Issue Articles Mass Spectrometry & Spectroscopy 67th ASMS Conference on Mass Spectrometry and Allied Topics Chromatography Using Gas Chromatography for measuring atmo...

View all digital editions


ACS National Meeting & Expo, Fall 2019

Aug 25 2019 San Diego, CA, USA

Microscopy Conference 2019

Sep 01 2019 Berlin, Germany

BMSS Annual Meeting

Sep 03 2019 Manchester, UK

JASIS 2019

Sep 04 2019 Chiba City, Japan

ICMGP 2019

Sep 08 2019 Krakow, Poland

View all events