Easy-to-Use RP Capillary Columns and Emitters Improve High-Resolution LC-MS Analysis

Proteomics, clinical peptides and biopharmaceutical research laboratories performing high throughput, high-performance liquid chromatography (HPLC), or liquid chromatography-mass spectrometry (LC-MS) analysis can now benefit from novel capillary chromatography columns and nano- and capillary emitters, specifically designed for highly sensitive top-down, middle-down analysis. These columns and emitters enable laboratories to achieve high-resolution, low-flow separations for intact proteins, monoclonal antibodies (mAbs) and peptides.

The Thermo Scientific MAbPac Reversed-Phase (RP) Capillary HPLC Column builds upon the separation power and reliability of the proven MAbPac RP chemistry in analytical flow. Together with the new Thermo Scientific EASY-Spray Nano and Capillary Emitters, the temperature-controlled MAbPac column enables maximum reliability and performance, while also providing excellent resolution, long column lifetime and low carry-over. The capillary format of the LC column improves sensitivity, achieving outstanding data results even with smaller sample volumes. The format makes the versatile column ideal for applications such as peptide mapping characterisation and analysis of mAbs fragments, antibody drug conjugates (ADC) and PEGylated proteins.

The MAbPac column is available in a convenient EASY-Spray design, which delivers proven ease-of-use by taking advantage of ‘plug and spray’ to low-flow LC. The standalone MAbPac RP Capillary LC column is designed with double nano viper fitters, positioned on each side of the column, enabling finger-tight grip to easily mount the column onto the system and eliminate dead volume. The nano- and capillary emitters are compatible for use with the Thermo Scientific EASY-Spray source and Thermo Scientific FAIMS Pro source. A simple hardware connection delivers continuous stable spray onto the MS system, enabling better reproducibility and robust nano- and capillary flow into LC-MS systems.

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