Microscopy & Microtechniques
Accurate Detection of Proteins Stained with Flamingo Fluorescent Dye
Jan 22 2007
Syngene's technical team used Dyversity, which consists of a light tight darkroom containing a 16-bit CCD camera fitted with a Cy dye lighting module, dual wavelength transilluminator, UV, long pass and Cy3 dye emission filters, to image 1D acrylamide gels. These gels, containing 1000-0.1ng of molecular weight standard PeppermintStick? (Invitrogen) stained with Flamingo, were imaged under three different conditions: Cy2 excitation with Cy3 emission filter; Cy2 excitation with UV emission filter and medium-wave UV excitation with a short pass emission filter.
Syngene's technical experts saw Dyversity could image 5ng of Flamingo stained protein in 2 seconds using medium-wave UV excitation and short pass emission filter. However, using Cy2 illumination with the Cy3 or UV emission filter, Dyversity was 10 times more sensitive, detecting as little as 0.5 ng of protein in the same time.
Dyversity can quickly image such minute amounts of Flamingo stained protein because its high quality camera has the fastest capture times per channel for fluorescent and visible dyes of any current CCD based system. Dyversity's darkroom also has a large door opening to accommodate a range of gel sizes and users can easily add new filters and lighting as they need to, making this the most versatile image analyser available.
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