• Optimised reagents for automated qPCR workflows


Optimised reagents for automated qPCR workflows

Feb 10 2024

In response to the increasing demand for stable reagents in automated qPCR workflows, Thermo Fisher Scientific introduces the Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix. Unlike conventional mixes, this innovative solution maintains peak performance at room temperature for over 8 hours, offering labs greater flexibility in incorporating 1-Step RT-qPCR into their automated processes.

Tailored for biopharma and clinical diagnostics labs, the TaqPath DuraPlex Master Mix provides a rapid, sensitive, and reproducible method for high-throughput molecular diagnostics and bioprocess quality control assays. Its benchtop-stable, single-tube design is optimised for detecting virus and bacterial pathogens, even in the presence of challenging PCR inhibitors. Labs can confidently automate their 1-Step RT-qPCR workflows without compromising sensitivity, specificity, or reproducibility, with the added capability to multiplex up to 6 targets in a single reaction.

Alison Todd, Chief Scientific Officer & Founder of SpeeDx Pty Ltd, expressed satisfaction with the product's performance, noting significant improvements in inhibitor tolerance and in-channel multiplexing, especially at low copy numbers.

The 1-Step RT-qPCR Master Mix from Thermo Fisher Scientific presents several advantages for laboratories. It boasts improved handling with lower viscosity and flexible characteristics for ease of use and storage, enhancing automation performance and compatibility. Moreover, the master mix instills confidence by demonstrating high tolerance to qPCR inhibitors, such as VTM, stool, blood, and urine, ensuring reliable assay performance even with challenging sample types. To guarantee reproducibility, the product is manufactured in an ISO 13485-certified and FDA-registered facility, with all lots undergoing functional testing for consistent and reliable performance.

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