Bonded Phases for Polar and Non-polar Molecules

The Cogent TYPE-C™ silica bonded phases, manufactured by MicroSolv Technology Corporation and available through Hichrom Ltd, have a surface populated by silicon-hydride functional groups instead of silanols. Patented bonding technology is then used to produce the Cogent Bidentate C18, Bidentate C8, UDC-Cholesterol, Phenyl and Diamond Hydride (the ‘Metabolomics Column’) phases, with direct silicon-carbon bonds on the silica hydride surface. These bonded phases have the ability to retain polar solutes at high concentrations of organic solvent by aqueous normal-phase (ANP). In addition, non-polar compounds can also be retained on the same column under reversed-phase conditions. The lack of surface silanols leads to fast equilibration times, excellent peak shape and extended column lifetimes for the assay of a wide range of pharmaceuticals, organic acids, carbohydrates, biochemicals, peptides, metabolites and many other compounds.