Chromatography
Published over 14 years ago. See the latest and most current information on Chromatography.
JPP Chromatography, a small UK technology company, announces the development of an air stable, single (one bottle) ‘hydrindantin-free’ ninhydrin reagent for amino acid analysis. All commercially available ninhydrin reagents sold for high performance ion chromatography amino acid determinations are currently based on the original 60 year old Moore and Stein method, which requires the presence of the highly sensitive hydrindantin as an ‘accelerator’. Two or more reagents must be mixed before use in the post-column reactor, where air must be rigidly excluded at all times - a tedious and inefficient process.
JPP’s reagent still causes a high speed reaction with amino acids and amines in the reaction coil at 130ºC to produce the intensely coloured Ruhemann’s purple. It can therefore be used on all ninhydrin based amino acid analysers without modification. It is totally unaffected by the presence of air, production is much cheaper, it is extremely stable and shelf- life is very long.
JPP’s over-riding aim was to come up with a product that makes life much easier for the end user, which it has succeeded in achieving. JPP seeks approaches from potential partners.
ILM 51.5 July 2026