Chromatography

Analysis of Monoclonal Antibody Charge Variants by Capillary Zone Electrophoresis

Sep 03 2012

Author: Marcia R Santos on behalf of Beckman Coulter Ltd

Free to read

This article has been unlocked and is ready to read.

Download

Charge heterogeneity analysis is important in the characterisation of monoclonal antibodies because it provides important information about product quality and stability. Heterogeneity can be caused by such molecular adaptions as C-terminal lysine modification, deamidation, and post translational modification. One method for separating charge variants is capillary isoelectric focusing (cIEF) which provides information regarding isoelectric point variation for related molecular isoforms. cIEF requires electrophoretic separation in a coated capillary which helps suppress electro-osmotic flow (EOF) and prevents surface protein adsorption but can result in a fairly long cycle time and complex sample preparation.
We demonstrate here that by using a simple separation buffer system and a bare fused silica capillary, it is possible to obtain a highly resolved, reproducible separation of a representative monoclonal antibody in less than 12 minutes. We also demonstrate that while high resolution can be achieved using a short effective length (20cm), the fine structure of the monoclonal antibody used in this study can be revealed by increasing the effective length to 40cm.
 

Free to read

This article has been unlocked and is ready to read.

Download


Digital Edition

International Labmate Buyers Guide 2022

June 2022

In This Edition Evaluation of Recovery of Basic Analytes Using a Mixed-Mode Strong Cation Exchange SPE Microplate by HPLC-MS The Benefits of SEC-MALLS in Biopharmaceutical Applications...

View all digital editions

Events

AACC Annual Meeting & Clinical Lab Expo

Jul 24 2022 Chicago, IL, USA

ICMGP 2022

Jul 24 2022 Virtual event

ACS National Meeting & Expo, Fall 2022

Aug 21 2022 Chicago, IL, USA & Online

ACHEMA 2022 - NEW DATES

Aug 22 2022 Frankfurt, Germany

IMSC 2022

Aug 27 2022 Maastricht, Netherlands

View all events