Mass Spectrometry & Spectroscopy

Quantification of Fluticasone Propionate and Salmeterol Xinafoate in Plasma at the Sub pg/mL Level Using UPLC/MS/MS  

Jul 09 2012

Author: Joanne Mather, Kendon Graham, Diego Rodriguez Cabaleiro, Stuart Chadwick, Paul Rainville, and Robert Plumb on behalf of Waters Corporation

Free to read

This article has been unlocked and is ready to read.


Sensitivity has been the driving force in the bioanalysis and DMPK arena for the last 15-20 years. The introduction of tandem quadrupole ESI mass spectrometry into the bioanalysis laboratory in the early 1990’s saw a step-change in the sensitivity and specificity of bioanalytical assays. Prior to this achieving sensitivities in the ng/mL range required complicated method development, elegant LC methods, elaborate solid-phase or liquid-liquid extraction processes to process large volumes of sample and even chemical modification of the analyte to impart better detection characteristic. The introduction of ESI tandem quadrupole MS changed the face of bioanalysis. Now sensitivities in the ng/mL range or could be achieved on virtually every molecule, from just a few hundred microlitres of sample without the need for complex sample preparation or derivitisation. Twenty years on and the pace of MS innovation and chromatographic science development means that sensitivities in the pg/mL range or below are now possible, even for the most challenging of molecules. 

Free to read

This article has been unlocked and is ready to read.


Digital Edition

Lab Asia 31.1 - February 2024

February 2024

In This Edition Chromatography Articles - Comparing techniques for flow rate measurement in Liquid Chromatography Mass Spectrometry & Spectroscopy Articles - APGC: A Better Future with...

View all digital editions


IFPAC 2024

Mar 03 2024 Bethesda, MA, USA

SIAF Guangzhou

Mar 04 2024 Guanghzou, China

China Lab 2024

Mar 05 2024 Guangzhou, China

Laborama 2024

Mar 14 2024 Brussels, Belgium

MSACL 2024

Mar 17 2024 Monterey, CA, USA

View all events