An Alternative Solid Phase Affinity Matrix for Chromatin Immunoprecipitation

The Porvair Chromatrap^® Pro-A, the novel solid-based matrix for chromatin immunoprecipitation assays (ChIP) has been successfully used by researchers to understand the role of CTCF, a transcription factor, in tumorigenesis in clinical tissue specimens.

A recent study by the School of Biological Sciences, University of Essex, used Chromatrap^® Pro-A 1ml columns with gravity feed to assess the occupancy of various key regulators such as c-myc and ERG1 at CTCF target sites in the p14ARF and Bax promotor regions in clinical tissue samples. Excellent enrichment was achieved from the complex tissue samples and the Chromatrap® Pro-A 1ml columns proved easy-to-use with either gravity or vacuum for more

rapid throughput. Full details are available in a newly published Application Note.

For a copy of the Application Note please visit http://www.chromatrap.com/uk/technical-references.