New ELISA Fluorescent 90-Minute Assay Announced

CatchPoint® SimpleStep® ELISA (SSE) kits from Abcam provide a simple and fast protocol with a single-wash step, assay time of 90 minutes or less, and a fluorometric readout that delivers an extended dynamic range.

Developed using Abcam’s SimpleStep ELISA technology and Molecular Devices’ CatchPoint fluorescent substrate, the addition of these kits to Abcam’s SimpleStep portfolio means that researchers can now choose from both fluorometric and colorimetric kits based on their experimental needs, with high-quality and highly-specific Abcam antibodies.

CatchPoint’s fluorescent substrate provides significantly improved linearity over an extended dynamic range when compared to TMB substrate. This extended dynamic range at both the lower and the upper end of readout curves reduces the need for sample dilutions, and allows quantitation at lower protein concentrations, improving sensitivity. The CatchPoint SSE kits are also validated across a wide range of biological samples and optimised for Molecular Devices microplate readers, providing reproducible results across many different undiluted sample types on industry-leading instrumentation.

CatchPoint SSE kits employ highly-validated antibodies, the majority of which have been developed using Abcam’s recombinant monoclonal antibody technology. Recombinant antibodies provide specificity and lot-to-lot reproducibility for consistent experimental results.

CatchPoint SSE as well as SimpleStep colorimetric kits, serve a wide range of interests, including cancer, neuroscience, cardiovascular and immunology research. These kits are available through the Abcam website and authorised distributors.