Hydrocell Reversed Phase Columns RP 10D and RP 5D for Direct High Molecular Weight of Protein and Enzyme Analyses and Purification

BioChrom Labs, Inc. provides innovative macroporous polymer beads (RP 10D and 5D) for direct protein analysis and purification. Hydrocell Reversed Phase Columns produced from high cross-linked, high pore volume, macroporous PS-DVB polymer beads that can be used to analyze and separate single amino acids, polypeptides and high molecular weight proteins and enzymes of up to more than 290 KDa molecular weight in a single column with high resolution, and reproducible chromatograms.

The gradient profiles of RP 10D and RP 5D are similar to gradient profiles in the conventional Silica based C-18 columns. It is easy during the method development to use Hydrocell Reversed Phase columns for biomolecule separation and analysis, especially for the high molecular weight of proteins and enzymes. Under the What is new section of the BioChrom Website, www.biochrom.com, you can see the demonstrating chromatograms using the RP 5D column to separate small peptides from 2-10 amino acids, and to analyze commercial human insulin from Novolog FexPen and bovine insulin.

You can also see the demonstrating chromatograms by using the RP 10D column to separate standard proteins molecular weight from 12.3 KDa. to 44.3 KDa., to separate high molecular weight of proteins and enzymes from 12.3 KDa. to 290 KDa., and to purify Thyroglobulin, molecular weight 670 KDa. The internal diameter will be available from 1mm microbore column to 50 mm preparative column and column length will be available from 35mm to 250 mm. Recently, the processes of producing Hydrocell macroporous polymer beads have been modified to improve their rigidity, stability and consistency.

Biochrom 2010 Catalog Download it HERE