How to Avoid Residual Solvent when Drying Reverse Phase HPLC Fractions

Traditional concentration of reverse phase HPLC fractions to dryness in a Genevac HT evaporator is a fast and safe process. However, for certain compounds a fully dried result can be hard to achieve due to compound interactions with water boosting up the boiling point making drying difficult.

Driven in part by the compound handling groups rejecting samples that were not completely dry, Genevac responded with the development of a fast lyophilisation process, or Lyospeed™, (a hybrid drying process combining conventional centrifugal evaporation and lyophilisation). Customer feedback suggests that in up to 90% of cases, depending on the compound properties, the application of Lyospeed™ produced a dry powder rather than the traditional thin film, making downstream processing far easier since compounds could either be more easily removed from the tube of vial, or re-dissolved in a suitable solvent. 

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