Now available from AbD Serotec, this valuable 84-page publication describes the principles behind HuCAL recombinant antibodies, the basis of their exceptional specificity, and presents protocols, examples, and troubleshooting tips for their use in applications such as Western blotting, ELISA, IHC, IP and flow cytometry.
HuCAL technology has been revolutionising the way monoclonal antibodies are generated for many years, and offers many advantages over traditional animal-based methods including speed, specificity, and production technology. HuCAL antibodies are selected from a high-quality, synthetic library of more than 15 billion expressible antibody specificities. This is the very latest and most powerful library created by MorphoSys, and has already generated thousands of monoclonals for researchers worldwide.
In addition to explaining the key design and construction features of the HuCAL library, the Technical Manual also explains why HuCAL antibodies are preferable to full IgGs for IHC, cell isolation, and even protein crystallization; how the selection can be geared to directly generate a monoclonal antibody that can distinguish between a wild-type protein and a single amino acid mutation; and why using the AgX? Antigen Expression Service can lead to increased specificity and functionality for your antibodies.
Copies of this essential publication for all antibody users are shipped with HuCAL antibodies, can be downloaded or requested as a hard copy from AbD Serotec.
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