UltraViolet Dyes for Multiplex Flow Cytometry
Jan 31 2023
StarBright Dyes from Bio-Rad Laboratories, Inc provide greater flexibility in multicolour flow cytometry panels. The StarBright Violet 760, StarBright UltraViolet 575, and StarBright UltraViolet 605 Dyes offer improved brightness with narrow excitation and emission profiles for precise resolution.
The Bio-Rad range of StarBright Dyes provides researchers with highly stable, validated flow antibodies conjugated to proprietary fluorescent nanoparticles and specific to key immunophenotyping targets, supporting multiplex flow cytometry panels in immunology research. Minimal lot-to-lot variation ensures reproducible and consistent staining, and the dyes are resistant to photobleaching and do not lose signal in fixation. The dyes, excited by violet (405 nm) and ultraviolet (355 nm) lasers, are compatible with the Bio-Rad ZE5 Cell Analyzer and S3e Cell Sorter, as well as most flow cytometers and a variety of experimental protocols, without the need for special buffers.
“The continued expansion of the Bio-Rad StarBright Dye portfolio, currently totalling 17 dyes, nine of which are StarBright Violet Dyes, gives researchers greater flexibility and choice when designing flow cytometry panels, by providing high-performance dyes excitable at a range of wavelengths,” said Mike Blundell, PhD, Bio-Rad Product Manager, Flow Cytometry, Life Science Group. “The exceptional brightness and unique profile of the dyes delivers enhanced resolution of rare populations and low-density antigens.”
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