Duetta™ - The Future of Fluorescence
Nov 18 2020 Read 299 Times
The HORIBA Duetta two-in-one spectrometer is revolutionising fluorescence spectroscopy. It can be used as a fluorometer, a UV-Vis-NIR spectrometer to measure absorbance, or a system that measures true molecular fingerprints via the simultaneous acquisition of fluorescence and absorbance.
The fluorescence spectra are acquired in a single shot by a multichannel CCD detector without time-consuming wavelength-by-wavelength scanning. Thus, a full-range spectrum can be generated in as little as 50 ms, more than 100x faster than typical fluorescence systems. This speed is a distinct advantage when collecting Excitation Emission Matrices (EEMs), i.e. multiple emission spectra at a range of excitation wavelengths. The CCD detector has the added advantage of a wider wavelength range than normally found on a bench-top instrument, extending 1,100 nm.
With this single system a variety of measurements are possibly, including fluorescence emission, fluorescence excitation, fluorescence kinetics (intensity versus time), fluorescence EEM, absorbance and % transmittance. A collection of Apps within the EZspec software translate these into tangible results too for example, to build concentration curves, provide an easy, customisable pass/fail routine, or determine protein concentration using the A280 methodology.
Not only does this two-in-one system offer all of this analytical capability within a single system, but also its dual nature ensures reliable and correct data by automatically correcting for Inner Filter Effects (IFE). Without this, fluorescence data is prone to error!
Inner Filter Effects (IFEs) can significantly affect fluorescence emission spectral profiles, distorting their general shape, shifting the spectral position of the peak maxima and decreasing the emission intensities. Thus, when recorded with a standard spectrofluorometer, the detected fluorescence does not coincide with the true fluorescence emission of the sample, even for small fluorophore concentrations. These fluorescence spectra distortions can be critical in display, lighting and bioimaging applications where reliability and a precise knowledge of the emission properties are required.
Such perturbations are observed with all spectrofluorometers. The Duetta, however, with its dual fluorescence and absorbance is able to measure true molecular fingerprints, correcting for IFE in real time…all in the blink of the eye!
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