Expansion of qPCR Offering for Screening Applications

Integrated DNA Technologies (IDT) has added additional flexibility to its PrimeTime™ qPCR product offering. In order to provide even greater user input, customers can now customise the Standard and XL qPCR Assays by specifying the dye and quencher pair used on the probe and the ratio of primer to probe. These new PrimeTime scale features work in combination with the existing versatility offered across the PrimeTime range, including the ability to set the design parameters, select exon spanning sites to reduce genomic contamination, and view the primer and probe sequences prior to purchase. In addition, the new features give researchers more flexibility for multiplex design and experimental setup. As a result, the final product can meet all personal and experimental preferences.

PrimeTime qPCR assays are 5’ nuclease assays consisting of a forward and reverse primer along with a dual-labelled probe. The assays are shipped lyophilised with all components in a single tube and with enough

reagents for 500 (Standard) or 2500 reactions (XL). The use of a fluorescent probe, as opposed to an intercalating dye, eliminates inaccuracies associated with primer dimers or non-specific binding. Fluorescence

is detected only when the probe is cleaved by the polymerase action of the elongation phase. This improved specificity ensures that users can gain precise and reproducible results with every reaction.