Laboratory Products
New Medium for Presumptive E. Coli Enumeration in Milk
Sep 29 2008
The key to E. coli enumeration is the addition of 4-methylumbelliferyl-β-D-glucuronide (MUG) to the standard Lauryl Tryptose broth formulation.
Used in conjunction with the Most Probable Numbers (MPN) technique, Modified Lauryl Sulphate Tryptose Broth with MUG & Trytpophan (ISO) allows presumptive E. coli to be enumerated from presumptive coliforms. The addition of MUG allows the positive discrimination of E. coli strains. The majority of E. coli produce β-glucuronidase enzyme which enables them to hydrolyse MUG, resulting in the release of a fluorogenic compound. Tubes which fluoresce under UV light are confirmed for E. coli by a positive indole reaction when Kovac`s reagent is added to the tube.
Modified Lauryl Sulphate Tryptose Broth with MUG & Tryptophan (ISO) is described in ISO 11866-1:2005 and is used in the HPA National Standard Method D5 for Enumeration of coliforms and presumptive Escherichia coli by the Most Probable Number (MPN) technique.
More Information
Digital Edition
Lab Asia 31.2 April 2024
April 2024
In This Edition Chromatography Articles - Approaches to troubleshooting an SPE method for the analysis of oligonucleotides (pt i) - High-precision liquid flow processes demand full fluidic c...
View all digital editions
Events
Microbiology Society Annual Conference 2024
Apr 08 2024 Edinburgh 2024
Apr 09 2024 Munich, Germany
Apr 10 2024 Helsinki, Finland
Analytica Anacon India & IndiaLabExpo
Apr 15 2024 Mumbai, India
Apr 16 2024 Moscow, Russia