Technique for Multi-Colour Bacterial Counting

UVP, LLC defines a technique to count multiple colour bacteria colonies with the ColonyDoc-It Imaging Station. The station captures brilliant colour colony images; the software then differentiates between multiple colours when counting the colonies. The ColonyDoc-It can differentiate between b-glucuronidase positive (blue) bacteria and negative (yellow) bacteria. The results generated shows detailed counts of each colour and percentage of the population determined to be positive in b -glucuronidase.

Janelle Batman, UVP Product Manager stated: “The ColonyDoc-It’s high resolution colour camera and advanced software algorithms provide researchers with the means to finely differentiate among the myriad colours expressed by colonies.“

The ColonyDoc-It Station’s innovative design enables users to automatically count colonies quickly and accurately.

The station features: integrated high resolution digital colour camera for brilliant colour colony images; several light sources for illuminating a wide range of stained media; use of pour, spread and spiral plates and filters with sizes from 33 to 150mm; capture colony sizes as small as 0.08mm; count colonies automatically and generate detailed statistics; doors create a darkroom environment; GFP fluorescence with epi

blue light and optional GFP filter; and time lapse image capture and counting.

In addition to counting multicolour bacteria colonies, the ColonyDoc-It Station can be used in applications such as microbiology, quality control, environmental and pharmaceutical.