• Automated Scale-up System for Reversed Phase Purification Released  

Mass spectrometry & spectroscopy

Automated Scale-up System for Reversed Phase Purification Released  

Shimadzu has released its automated scale-up system named ASAPrep for RPC in reversed phase purification. ASAPrep is an acronym for ‘Automated Scale-up from Analytical to Preparative conditions’. It is an algorithm (patent pending) for calculation of optimal initial organic mobile phase concentration in preparative scale using the retention time acquired by LC-PDA-MS measurement and judging of the difference between success and failure of purification. Its calculation uses pre-registered ASAPrep operational expression on newly developed Open Solution Purification software.

ASAPrep for reversed phase purification (RPC) enables construction of a user-independent purification workflow. Based on an algorithm, the preparative method is created automatically, and users will save time and increase throughput. ASAPrep for RPC runs on an LCMS-2020 single quadrupole system in analytical and preparative mode.

A cycle from synthesis of New Chemical Entities (NCE) and purification to High Throughput Screening (HTS) needs to be turned around smoothly to enhance throughput in medicinal chemistry. In the case of NCE synthesis and HTS, its automation is recently advanced but the speed of purification does not match NCE synthesis and HTS.

Medicinal chemistry departments of large pharmaceutical industries are capable of synthesising hundreds of new compounds per day, but they cannot provide high purity compounds in sufficient amounts for HTS. This is because the quality of purification depends highly on the skills of the chromatographers.

Based on the requirements of Takeda’s researchers, Shimadzu and Takeda have jointly developed ASAPrep for RPC which can automatically predict success and failure of purification and determine an optimal preparative condition. Takeda is the largest pharmaceutical company in Japan and operates subsidiaries on all continents.

Dr Teruhisa Ueda, Managing Executive Officer, Director of Analysis and Measuring Business in Shimadzu, stated: “We are proud to contribute with a set-up of new purification workflow in Takeda’s chemistry department. We believe ASAPrep for RPC is a breakthrough in technology, and not only enables high throughput and automated purification, but also expands popularisation of reversed phase purification which has been avoided by researchers due to the hurdle of method development.”


Digital Edition

Lab Asia 32.1 Feb 2025

February 2025

Chromatography Articles - Comparing volumetric and thermal flowmeters for assessing and validating liquid chromatography performance Mass Spectrometry & Spectroscopy Articles - The importa...

View all digital editions

Events

SPS Smart Production Solutions Guangzhou

Feb 25 2025 Guanghzou, China

SmartLab Exchange

Feb 25 2025 Amsterdam, Netherlands

Nepal Lab

Feb 27 2025 Kathmandu, Nepal

PITTCON 2025

Mar 01 2025 Boston, MA, USA

IFPAC 2025

Mar 02 2025 Bethesda, MD, USA

View all events