Monolithic Chromatography and its Modern Applications
Perry G. Wang Hardback
Now available as an eBook from Amazon>>
High-performance liquid chromatography has become the predominant global separation technique over the past four decades. However, the recent development of an alternative column packing material - a one-piece porous solid known as a ‘monolith’ - has transformed the field. Monoliths have attracted a large amount of interest due to the enhanced speed and thoroughness with which they can separate complex mixtures of biological molecules.
Take a look at the book in a page turning digital version by clicking HERE>> - To view all the pages, the book needs to be ordered.
Monoliths are porous rod structures characterised by mesopores and macropores. These pores provide monoliths with high permeability, a large number of channels and a high surface area available for reactivity. The backbone of a monolithic column is composed of either an organic or inorganic substrate and can easily be chemically altered for specific applications. Their unique structure gives them several physico-mechanical properties that enable them to perform competitively against traditionally packed columns.
In this book, global experts comprehensively describe this developing field. Part I covers the synthesis and characterisation of the columns and Part II dicusses their numerous applications.
This volume will be of interest to those already using monolithic columns, as well as those who are new to the area and would like to know more about this technique.
Table of Contents
Part I Synthesis and Characterisation of Monolithic Columns
Chapter 1 Affinity Monolith Chromatography: Principles and Recent Developments
Michelle J. Yoo and David S. Hage
Chapter 2 Characterisation of Pore Structure and Its Impact on the Chromatographic Properties of Organic Polymer Monolithic Capillary Columns
Pavel Jandera and Jiri Urban
Chapter 3 Silica-based Porous Monoliths: Synthesis, Characterisation and Applications in Chromatographic Separation
Hua Zhong and Qihua Yang
Chapter 4 Preparation and Characterisation of Organic Polymer Monolithic Capillary Columns
Pavel Coufal, Zuzana Bosakova, Jakub Sirc, Vera Pacakova and Karel Stulık
Chapter 5 Ring-opening Metathesis Polymerisation- derived Capillary Monoliths: Versatile Analytical Tools for Modern Biomedical Research
Frank M. Sinner and Christina Gatschelhofer
Chapter 6 Monolithic Cryogel Beds Prepared Under Varying Freezing Temperatures and Their Application in the Isolation of Nucleotides
Kejian Yao, Junxian Yun and Shaochuan Shen
Chapter 7 Organic Monoliths as Stationary Phases in Chromatography
Andreas Greiderer, S. Clark Ligon Jr., Christian W. Huck and Gunther K. Bonn
Part II Applications of Monolithic Columns
Chapter 8 Applications of Monolithic Chromatography in Pharmaceutical Analysis
Debby Mangelings and Yvan Vander Heyden
Chapter 9 Monolithic Columns in Microscale Separations
Ren’an Wu and Hanfa Zou
Chapter 10 Monolithic Ion Exchange Phases for the Separation of Small Inorganic and Organic Ions
Brett Paull and Damian Connolly
Chapter 11 Monolithic Column Chromatography Coupled with Chemiluminescence Detection
Paul S. Francis and Jacqui L. Adcock
Chapter 12 Fast Separations of Phytochemicals by High-performance Liquid Chromatography Using Monolithic Columns
Mauricio A. Rostagno and Ana Villares
Chapter 13 Monolithic Stationary Phases for Affinity-based Separations
Panagiotis Manesiotis and Georgios Theodoridis
Chapter 14 Immobilisation of Enzymes on Monolithic Matrices: Applications in Drug Discovery
Vincenza Andrisano and Manuela Bartolini
Chapter 15 Applications of Monolithic Chromatography in Support of Pharmaceutical Development of Drug Substances and Formulations
Naijun Wu and Zhong Li
Chapter 16 Use of Monolithic Material as On-line Extraction Support for High-throughput Liquid Chromatography Tandem Mass Spectrometry Analysis of Biological Samples
Raymond Naxing Xu, Grace E. Kim, Maria P. Metchkarova, Phillip Vaca, Matthew J. Rieser and Tawakol A. El-Shourbagy
Chapter 17 Convective Interaction Media Monoliths for Separation and Purification of pDNA and Viruses
Matjaz Peterka, Petra Kramberger and Ales Strancar
Chapter 18 Analysis of Methylated Arginines and Amino Acids Using a Monolithic Silica Column
Chapter 19 Applications of Monolithic Columns in the Analysis of Illicit Drug Samples
Kim McFadden and Brian Carney
Chapter 20 Applications of Monolithic Phases in Fundamental Studies of Vegetable Proteins and Food Analysis and Technology
J.M. Saz and M.L. Marina
Chapter 21 Toxicological Analysis Using Monolithic Silica Spin-column Extraction and Chromatographic Separations
Takeshi Saito, Akira Namera and Akihiro Nakamoto
Chapter 22 Amphiphilic Acrylamide-based Continuous Beds in Capillary Electrochromatography
Olga Kornysova, Audrius Maruska and Ute Pyell
Dr. Perry G. Wang received his BS degree in chemistry from Shandong University, China. He earned his MS and PhD degrees in environmental engineering from Oregon State University, USA.
He is currently a Research Chemist in the Office of Regulatory Science, Center for Food Safety and Applied Nutrition, US Food and Drug Administration (US FDA). His activities include analytical method development and validation for drugs and constituents in foods and cosmetic products using advanced instrumentation. His expertise focuses on high-throughput drug analysis by LC/MS/MS for the pharmaceutical industry.
Dr. Wang has been invited to prepare, organise and preside over symposia for the Pittsburg Conference (PittCon) and American Chemistry Society (ACS) Annual Meetings. He has been an invited speaker at numerous international conferences including PittCon, the Federation of Analytical Chemistry and Spectroscopy Societies (FACSS) Meetings, the Beijing Conference and Exhibition on Instrumental Analysis (BCEIA) and the International Symposium on Chemical Biology and Combinatorial Chemistry (ICCBCC). He also has been invited to teach short courses on high-throughput method development for drug analysis by LC/MS/MS at PittCon, ACS Annual Meetings and the Eastern Analytical Symposium (EAS).
In This Edition Chromatography - Achiral Supercritical Fluid Chromatography (SFC) for the Purification of Pharmaceuticals - Equivalent GC systems performance for regulatory method compliance...
View all digital editions
May 29 2022 Virtual event
May 31 2022 Frankfurt, Germany
Jun 05 2022 Minneapolis, MN, USA
Jun 08 2022 Rotterdam, Netherlands
Jun 08 2022 Helsinki, Finland