SEC-LS Dual Integrated Detector Dedicated to Proteins

Malvern Instruments has launched the Zetasizer μV with OmniFACE and OmniSEC, a new SEC-LS (size exclusion chromatography - light scattering) detector package dedicated to protein analysis.

The proven Zetasizer μV offers dual DLS (dynamic light scattering) and SLS (static light scattering) capabilities. It can be used as a stand-alone detector, analysing samples in a cuvette, and now as a fully featured modular chromatography detector. For protein applications, this makes the Zetasizer μV an ideal tool. Applications in stand-alone batch mode include the study of molecular size and formulation stability, and in SEC detector mode the measurement of molecular weight, determination of oligomeric state and detection and quantification of oligomers and aggregates. The absolute nature of the measurement means that no column calibration is required.

The Zetasizer μV is available as a complete chromatography system or can be added to existing SEC modules. Malvern’s OmniFACE provides the necessary connection that enables the Zetasizer μV to be linked into any third party SEC system and detectors, so that output signals can be used by the OmniSEC software for results calculation. Connecting the Zetasizer μV is simplicity itself. Using standard chromatography tubing it can be added in series with other detectors such as UV and RI. The laser stability and optical setup of the Zetasizer μV ensure excellent baseline stability and signal-to-noise for maximum sensitivity, while the 8μL flow cell minimises band broadening.