IC-MS Technique for Metabolomics Studies

Dionex is pleased to announce the release of a Customer Application Note (CAN) demonstrating the use of ion-exchange chromatography coupled with mass spectrometry for the separation for polar compounds present in biological samples. CAN 108: Ion Chromatography Coupled with Mass Spectrometry for Metabolomics demonstrates efficient separation of biological metabolites using ion chromatography with MS detection. Ionic and polar compounds such as organic acids, carbohydrates, nucleotides, and amino acids are difficult to separate using traditional reversed-phase columns. The technique presented in CAN 108 demonstrates effective separation of these compounds using ion-exchange followed by on-line desalting to allow mass spectrometric detection. By combining the best features of both techniques, this method provides ultralow levels of quantitation in a wide variety of biological matrices, including blood, urine, and sera.