New Columns Accelerate LC/MS Analysis of Synthetic Oligonucleotides

Phenomenex Inc introduced Clarity® Oligo-MS™ columns for rapid and efficient LC/MS characterisation and quality control of synthetic RNA and DNA. Based on the company’s core-shell particle technology, the new C18 columns deliver significantly shorter run times than traditional media, along with increased resolution and sensitivity. With the high resolving power of Clarity Oligo-MS, impurities in complex synthetic mixtures can be

separated from the peak of interest in less than 10 minutes.

These new Oligo-MS columns, which are part of the Clarity BioSolutions portfolio, are packed with either 2.6μm or 1.7μm particles, enabling high efficiency on any HPLC or UHPLC platform. The Clarity Oligo-MS 2.6μm columns operate at reduced backpressures compared to other oligonucleotide-specific columns, delivering UHPLC performance on any LC instrument, and methods are easily transferable. The Clarity Oligo-MS 1.7μm columns boost the performance of existing sub-2μm methods on high-pressure systems.

With traditional, fully porous particles, efficiency decreases as flow rate increases, resulting in loss of resolution and sensitivity and longer overall analysis time. The Phenomenex core-shell technology enables high resolution and sensitivity over an extended linear velocity without generating excessive backpressure. These columns provide roughly twice the efficiency of fully porous 3-micron columns and three times the efficiency of fully porous 5-micron columns, with significantly lower limits of detection and quantitation.