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Nov 07 2017
Chromatographic purification of natural compounds presents many challenges to scientists because of the complex nature of the starting matrices that are used in the process. These starting materials can damage traditional columns and cartridges, decreasing the length of their usage and increasing costs; that is, if the particular system can even accommodate the starting material. Centrifugal partition chromatography (CPC), which uses both liquid stationary and mobile phases, can handle heavily particulated, complex starting materials, such as direct extracts from many biological sources, and has been shown useful for the isolation of piperine from Piper nigrum1 and gingerol from ginger. Additionally, by relying on a liquid stationary phase, traditional columns and cartridges used by preparative HPLC and FLASH methods do not need to be replaced.
CPC is a cost effective alternative for these purification techniques and yields a high quantity of pure compounds. CPC is your ideal solution for natural extract fractionation, natural compound purification, and protein separations.
THE ADVANTAGES OF CPC
• High injection capacity
• No need for pre-treatment prior to injection
• No denaturation of fragile molecules
• No risk of a blocked or contaminated column
BENEFITS OF CPC VS. FLASH AND PREP HPLC
• Purity > 99% and recovery > 95%
• No sample loss • Cost effective - No expensive columns to replace
• Five times less solvent consumption
• High flow rate for low run times
• Easily scalable (milligrams to kilograms)
For further information on CPC please click here.
Digital Edition
International Labmate 48.6 - September 2023
September 2023
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