Agilent Technologies, Inc has introduced the Agilent High Sensitivity Protein 250 Kit for the Agilent 2100 bioanalyzer, which delivers more
sensitivity than silver-stained SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The new kit also provides quantitation capability along with the other advantages of a microfluidic lab-on-a-chip based kit.
The High Sensitivity Protein Kit, designed for both pharmaceutical QA/QC and life sciences research experiments, detects proteins down to as little 1 pg/ul, comparable to or better than silver-staining slab gels. The kit features a dynamic range of four orders of magnitude for
quantitation and covers a sizing range from 10 - 250 kDa. For QA/QC applications, users can detect a 0.05-percent impurity while visualising the main target with reliable quantitation.
The new kit features a direct labeling reaction that is highly reproducible. Separation, quantitation and purity measurements can be performed in a single step, and typical throughput for QA/QC is
10 samples per hour.
Each High Sensitivity Protein 250 kit can be used to analyse 100 samples. It includes labelling dye and reagents, 10 Microfluidic chips (each capable of running 10 samples), bioanalyser separation reagents,
and user documentation. The assay runs on the standard Agilent 2100 bioanalyzer system.
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