Increased Sensitivity, Improved Resolution and Faster Analysis Times For Compendial Methods
Dec 23 2009 Read 1252 Times
Author: Elli Abbasi, Jeff Layne, Heiko Behr, and Sky Countryman on behalf of Phenomenex Inc
Determination of Impurities and Related Substances Using Newly Developed 2.6 μm Core-Shell Kinetex™ LC Columns
Presently, HPLC methods for the determination of impurities and related substances of drug products specified in monographs by the various Pharmacopoeial agencies typically employ LC columns packed with fully-porous 3 or 5 micron spherical silica chromatographic media. Due to the performance limitations of fully-porous 3 and 5 micron spherical silica chromatographic media, these analytical methods commonly require long analysis times.
Additionally, accurate quantitation of low-level impurities in routine LC-UV applications may be challenging due to the low intensity peaks generated by these columns. In recent years, smaller fully-porous LC particles
(sub-2 micron diameter) have been introduced that offer faster analysis times and generate higher intensity peaks for better sensitivity. Unfortunately, widespread adoption of this sub-2 micron HPLC column technology has been slowed since the smaller particle columns generate system backpressures that require specialised ultra-high pressure capable LC instrumentation.
Do you like or dislike what you have read? Why not post a comment to tell others / the manufacturer and our Editor what you think. To leave comments please complete the form below. Providing the content is approved, your comment will be on screen in less than 24 hours. Leaving comments on product information and articles can assist with future editorial and article content. Post questions, thoughts or simply whether you like the content.
In This Edition Articles - Expanding the Boundaries of Light Scattering for Macromolecules - Electrostatic Charges and Their Effects on Weighing - How the world’s most advanced analytical...
View all digital editions
Mar 03 2021 Guanghzou, China
Mar 08 2021 Virtual Event
Mar 22 2021 Digital event
Apr 14 2021 Tokyo, Japan
Apr 20 2021 Virtual event