HILIC Columns for the Separation of Polar Molecules

Nacalai Tesque offers a novel triazole bonded silica stationary phase which is designed for the HILIC separation of polar molecules. This triazole bonded phase offers higher polarity than non-modified silica columns, which are commonly used for HILIC, resulting in stronger hydrophilic interactions. The positively charged triazole stationary phase also offers an anion-exchange mechanism, enabling the strong retention of acidic compounds. The 2 separation modes, HILIC and ionic interaction, can be controlled by varying key eluent parameters such as pH, concentration of organic solvent and buffer ion strength. COSMOSIL HILIC is available with both 5 and 2.5 micron particle sizes. A COSMOSIL HILIC Application Notebook, containing about 200 chromatograms for the separation of polar compounds using COSMOSIL HILIC columns also available. For more information or to request a copy of the COSMOSIL HILIC Application Notebook contact Hichrom Ltd.