Laboratory Products
Microarray Cross-platform Study
Jun 10 2008
engineered DNA targets demonstrated the high performance and cost effectiveness of Agilent microarrays.
Unlike previous evaluations, the composition of the spike-in DNA in this study was engineered to mimic chromatin immunoprecipitation (ChIP) or copy-number amplification experiments across a wide dynamic range. To ensure a fair evaluation, the composition was not disclosed to participants. This made it possible to quantitatively evaluate each platformâs sensitivity and specificity of detecting and quantifying a predefined standard without bias.
First, platforms were compared using their highest possible tiling density. In evaluating results from comparable algorithms, Agilent
consistently achieved the highest or equivalent scores, but did so with fewer probes, half as many replicates and less sample DNA than either of the other platforms.
Overall, the study found that longer oligonucleotide (60-nt) microarrays, such as Agilentâs, were more sensitive at detecting very low enrichment or copy number. Additionally, Agilent demonstrated the highest levels of sensitivity and specificity per probe, in some cases
by orders of magnitude, over a range of simulated tiling densities.
Digital Edition
Lab Asia 31.2 April 2024
April 2024
In This Edition Chromatography Articles - Approaches to troubleshooting an SPE method for the analysis of oligonucleotides (pt i) - High-precision liquid flow processes demand full fluidic c...
View all digital editions
Events
May 05 2024 Seville, Spain
InformEx Zone at CPhl North America
May 07 2024 Pennsylvania, PA, USA
May 14 2024 Oklahoma City, OK, USA
May 15 2024 Birmingham, UK
May 21 2024 Lagos, Nigeria