The Next Stage of PCR
Oct 09 2017 Comments 0
The new Mastercycler X50 is the elegant synthesis of flexibility for research
applications and standardisation for routine applications such as food testing etc. A new highly intuitive touch screen concept puts all of those benefits always at your fingertips.
Speed and enhanced optimisation functions like the 2D-Gradient make the Mastercycler X50 the ideal tool for advanced research in molecular biology. The excellent block temperature control and regulation give rise to the next stage of optimisation, whereas the adaptable user management and profound documentation capabilities give peace of mind to laboratories working or
conforming to set standards.
A highly intuitive touch display, low noise levels, low power consumption, and the versatile flexlid lid concept complete the product to be a powerful yet discrete tool. Up to 10 units can be combined – ideal for high throughput applications or labs with a high number of users running different assays. Should you feel you need more flexibility or throughput, up to 50 units can be
combined in a computer-controlled network.
Optimising the annealing temperature to improve PCR results is a routine task, so why not optimise as far as you can? More and more findings indicate that optimisation of the denaturation temperature is worthwhile as well. A high denaturation temperature will harm the enzyme and other biomolecules in your assay. A low denaturation temperature may result in inefficient splitting of the complimentary DNA strands, as is often found with GC-rich templates or templates prone to form ‘hairpin structures’.
Eppendorf’s new 2D-Gradient allows optimisation of the annealing and
the denaturation temperature in a single run – taking less time than ever before. This gives you the ideal set of temperatures for your PCR assay - quickly, conveniently, reliably.
There ia a large variety of cyclers available with different ramp rates published. If you compare the corresponding run times by performing the same protocol using different cyclers, you see that ramp rates do not tell the full story about whether a cycler is fast or not.
The better you can control the reproducibility of your PCR, the easier it is to run it consistently and to document the reproducibility of your workflow. Excellent block homogeneity, accuracy and precision as well as regular
temperature verification, stringent user management and advanced connectivity are cornerstones of a validated PCR workflow. The Mastercycler X50 supports your needs for instrument qualification and method validation with the following features:
- excellent block homogeneity (0.2ºC at 20–72ºC)
- excellent block accuracy and precision (0.15ºC)
- fast temperature verification possible
- adjustable verification settings – according to your audit needs
- advanced documentation capabilities
- adjustable user management – from flexible to strict
- transparent performance data
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In this Issue Articles - How to Achieve Ultimate PCR Optimisation Spotlight Features - Incubators, Freezers & Cooling Equipment - Balances & Strain Gauges - Proteomics, Genomics & Mic...
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